Journal: BMC Oral Health

Article Title: Effects of calcium-containing solutions on fluorescence-detectable plaque area and enamel mineral volume: an in vitro and exploratory clinical study

doi: 10.1186/s12903-026-09789-z

Figure Lengend Snippet: In vitro effects of calcium solutions on QLF-area and MV changes. A SEM images of tooth occlusal lesions before and after calcium solution treatment (Red box and blue arrow indicate the area where the 130× magnification pictures are shown; Red arrows indicate the lesion with the demineralization). B Micro-CT images of teeth in four solution groups, while the threshold-segmented enamel mineralized volumes (MVs) were analyzed and compared quantitatively before and after rinsing (Red arrows indicate the demineralization lesion; AS + Calcium: the group of combination of artificial saliva and calcium solution). C QLF images of teeth in four groups, with quantitative analysis of QLF-areas (Red fluorescence indicates plaque-associated fluorescence detected by QLF). (Box plots indicate the median, interquartile range, whiskers (minimum to maximum), and individual data points; n = 5 per group; Wilcoxon matched-pairs signed-rank test in B and C ). D Correlation between ΔQLF-areas and ΔMVs across groups (Spearman’s rank correlation. n = 5 per group; AS + Calcium: the group of combination of artificial saliva and calcium solution). E Correlation between ΔQLF-area and ΔMV across all in vitro treatment groups. The association was assessed using Spearman’s rank correlation. Individual data points are shown; n = 20 total. Spearman’s ρ = −0.7710, p < 0.0001. The fitted lines in D and E are shown only to visualize the direction of the association

Article Snippet: Micro-computed tomography (micro-CT) images were acquired using a Quantum GX2 micro-CT system (PerkinElmer, Waltham, MA, USA) at 90 kV and 160 μA, with a 4.5-min scan time.

Techniques: In Vitro, Micro-CT, Analysis, Fluorescence